General Cell Lines and Cell Cultures
Lonza Walkersville AD2 4D-Nucleofector Y Kit
Electroporation-based methods have so far required cells to be in suspension for transfection However the 4D-Nucleofector Y Unit allows direct Nucleofection of cells in adherence Cells which typically grow in adherence in cell culture can be kept and transfected by Nucleofection in their physiological state The AD2 Primary Cell 4D-Nucleofector Y Kit is one of our two kits that are suited for electroporation of primary cells in adherence when working with the 4D-Nucleofector Y Unit The cells are cultured in the 24-well culture plates provided with the kit and for the transfection step a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific Nucleofector Solution mixed with the DNA or RNA substrate
Eurofins Discoverx PathHunter CHO-K1 GPR139 β-Arrestin Cell Line, 2 vials. (93-0954C2)
The PathHunter GPR139 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. TC-O 9311) based activation of GPR139 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR139 and EA-tagged β-Arrestin-2. Activation of GPR139 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mPPYR1 (mNPY4R) β-Arrestin Cell Line, 2 vials. (93-0774C2)
The PathHunter mPPYR1 (mNPY4R) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. pancreatic polypeptide) based activation of mPPYR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPPYR1 and EA-tagged β-Arrestin-2. Activation of mPPYR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 5 tissue spots, 5 slides/PK
BioChain's FFPE multiple tissue panels with different types of tumors are designed for screening gene expression in multiple tumor tissues. Each tumor tissue is paired with a corresponding normal control tissue. A licensed pathologist issued a pathological diagnosis of each tumor based on examination of tissue sections. Documentation of tissues' clinical histories is available upon request. mRNA Northern blot and Western blots as well as total RNA Norterhn blot are available in identical formats.
Cayman Chemical OxytocIn PolycLNal ntIsRum
Immunogen Bovine collagen type IIA Host Chimeric monoclonal antibody Species Reactivity ( ) Human mouse bovine Cross Reactivity ( ) Citrullinated form of the triple helical collagen type II C1 epitope (amino acids 359-369) denatured collagen type II denatured collagen type II triple helical C1 epitope (amino acids 359-369). Some cross reactivity to non-citrullinated epitopes on collagen type II Applications ELISA IHC
Eurofins Discoverx PathHunter U2OS BLK Activity Assay, 2 vials. (93-0882C3)
The PathHunter U2OS BLK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the BLK kinase domain. The cell line is engineered to express the catalytic domain from BLK fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the BLK catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter Jurkat PD-1 (SHP1) Signaling Assay, 2 vials. (93-1104C19)
The PathHunter PD-1 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. PD-L1, PD-L2) based activation of PD-1 receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PD-1 target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PathHunter CHO-K1 mFPR2 β-Arrestin Cell Line, 2 vials. (93-1034C2)
The PathHunter mFPR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. formylpeptides) based activation of mFPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mFPR2 and EA-tagged β-Arrestin-2. Activation of mFPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS p53 Nuclear Translocation Cell Line, 2 vials. (93-0757C3)
The PathHunter U2OS p53 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the p53 DNA damage pathway. This cell line enables assessment of ligand (e.g. camptothecin) based pathway activation via detection of resulting p53 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged p53 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of p53 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Bioss Progesterone Receptor - Ser294
Progesterone Receptor (Ser294) Primary Antibody Unconjugated Host Species Mouse Target Species Human Clonality Monoclonal Applications WB IHC 608Concentration Lot Dependent Size 100 ul Storage Buffer 10 mM HEPES (pH 7 5) 150 mM NaCl 100 g per ml BSA and 50% glycerol
Eurofins Discoverx PathHunter CHO-K1 GPR135 (HUMNPIIY20) β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0431C2A)
The PathHunter GPR135 (HUMNPIIY20) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR135 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR135 and EA-tagged β-Arrestin-2. Activation of GPR135 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS IL18R1/IL18RAP Dimerization Cell Line, 2 vials. (93-1131C3)
The PathHunter IL18R1/IL18RAP Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-18) induced dimerization of IL18R1/IL18RAP receptors. This product uses EFC technology. The cell line included overexpresses IL18R1 target protein fused to PK alongside IL18RAP target protein fused to EA. Upon ligand-induced dimerization of IL18R1/IL18RAP, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Eurofins Discoverx PathHunter U2OS cINSRa Functional Assay, 2 vials. (93-1016C3)
The PathHunter U2OS cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS c-MET/c-MET Dimerization Cell Line, 2 vials. (93-1063C3)
The PathHunter c-MET/c-MET Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. hepatocyte growth factor) induced dimerization of c-MET/c-MET receptors. This product uses EFC technology. The cell line included overexpresses c-MET target protein fused to PK alongside c-MET target protein fused to EA. Upon ligand-induced dimerization of c-MET/c-MET, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.